Denaturation of Bacillus Amyloliquefaciens α-amylase with Urea

Thermostable α-amylase from Lignocellulosic Residues Using Bacillus amyloliquefaciens

thermal denaturation of α-amylase from bacillus amyloliquefaciens in the presence of sodium dodecyl sulphate*

the thermal denaturation of α-amylase from bacillus amyloliquefaciens has been investigated in the presence and absence of sodium dodecyl sulphate (sds) over the temperature range (293-373) k in 20 mm sodium phosphate buffer, ph 6.9, using temperature scanning spectroscopy. the presence of sds caused the destabilization of α-amylase resulting in a decrease in the temperature of unfolding with a...

Microbial interactions for enhancement of α-amylase production by Bacillus amyloliquefaciens 04BBA15 and Lactobacillus fermentum 04BBA19

Interactions occurring between Saccharomyces cerevisiae and two thermostable α-amylase producing strains (Bacillus amyloliquefaciens 04BBA15 and Lactobacillus fermentum 04BBA19) were analyzed by comparing their growth patterns obtained in isolation with those obtained in mixture. The difference between the patterns was assessed using analysis of variance (ANOVA) in order to measure how much the...

Improved thermostable α-amylase activity of Bacillus amyloliquefaciens by low-energy ion implantation.

Thermostable α-amylase is of great importance in the starch fermentation industry; it is extensively used in the manufacture of beverages, baby foods, medicines, and pharmaceuticals. Bacillus amyloliquefaciens produces thermostable α-amylase; however, production of thermostable α-amylase is limited. Ion-beam implantation is an effective method for mutation breeding in microbes. We conduct...

Solid Culturing of Bacillus amyloliquefaciens for Alpha Amylase Production

Fourteen different agroresidues were screened for alpha amylase production using Bacillus amyloliquefaciens ATCC 23842. Among them, wheat bran (WB) and groundnut oil cake (GOC) in mass ratio of 1:1 was proved as the best substrate source. Supplementation with 0.01 M KH2PO4 and 1 % soluble starch enhanced the enzyme yield considerably. Maximum enzyme recovery from the solid mass was obtained whe...